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Reference #: 1006351
SHIFT:Day (United States of America)

Seeking Breakthrough Makers

Children's Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.

At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care-and your career.

CHOP's Commitment to Diversity, Equity, and Inclusion

CHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace. We are a team dedicated to fostering an environment that allows for all to be their authentic selves. We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.

We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.

A Brief Overview
The Xing laboratory at The Children's Hospital of Philadelphia and University of Pennsylvania seeks an experienced research assistant to assist in running a busy experimental laboratory, including conducting routine molecular biology experiments. Among other duties, the candidate will have specific responsibilities in generating numerous genomic and transcriptomic sequencing libraries. In this position, the candidate will have the opportunity to work with state-of-the-art next-generation sequencing (NGS) technologies, including but not limited to RNA-seq, single-cell RNA-seq, Oxford Nanopore long-read sequencing, and others. The candidate will be involved in all aspects of the lab's NGS workflows, developing new methodologies for these technologies, as well as implementing and troubleshooting NGS protocols.

The Xing laboratory studies splicing in human diseases, including cancer. Post-transcriptional RNA processing and modifications are important mechanisms for gene regulation and functional diversity in eukaryotic cells. The lab develops and applies high-throughput sequencing strategies and transcriptomic engineering technologies to study the regulation and function of RNA splicing in human diseases.

The ideal candidate will:

Assist in running a busy experimental laboratory, including maintaining mammalian cell cultures, performing molecular assays including molecular cloning, and keeping clear experimental records according to a specific format.
Communicate complex ideas and concepts to those with limited knowledge and understanding, as well as to peers.
Have a Bachelor's degree and at least 2 years of relevant experience or a Master's degree in Molecular/Cell Biology, Biotechnology, or a related discipline from a leading academic institution.
Have specific research expertise in the following areas are required or preferred: experience with handling RNA samples (required), NGS or third-generation sequencing experience(preferred), genomics studies(preferred).

What you will do
Independently perform advanced, complex research procedures on experimental samples
Formulate and perform lab experiments, analyze results, interpret methodology, report data, and maintain appropriate log books and lab notebook
Modify methods and protocols as required with minimum direction from PI/Sr. Scientist
Assist in development and troubleshooting of new techniques and ideas
Prepare lab reagents, monitor and maintain lab inventory
Supervise, train, and mentor junior and new staff members
Determine work assignments, schedules, and lab priorities
Present and discuss work at weekly lab meetings and national symposia
Assist in writing of grants, journal articles, poster, and oral presentations
Education Qualifications
Bachelor's Degree Required

Experience Qualifications
At least three (3) years of scientifically relevant experience Required
At least five (5) years of scientifically relevant experience Preferred

Skills and Abilities
Proficient in various research and/or analytical techniques required

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, professionals working onsite-at any CHOP location, for any portion of time-must be vaccinated for COVID-19.

CHOP also requires employees who work in patient care buildings to receive an annual influenza vaccine. Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.

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Independently perform advanced, complex research procedures on experimental samples
Formulate and perform lab experiments, analyze results, interpret methodology, report data, and maintain appropriate log books and lab notebook
Modify methods and protocols as required with minimum direction from PI/Sr. Scientist
Assist in development and troubleshooting of new techniques and ideas
Prepare lab reagents, monitor and maintain lab inventory
Supervise, train, and mentor junior and new staff members
Determine work assignments, schedules, and lab priorities
Present and discuss work at weekly lab meetings and national symposia
Assist in writing of grants, journal articles, poster, and oral presentations

Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.